Automated, High Throughput Methods for the Quantification of Endogenous Cellular Kinase Activity using HTRF Technology

This poster describes two methods using HTRF technology to quantify cellular kinase activity without overexpression of kinase or its substrate.

Two specific kinases were included in the study:
1. Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), one of multiple subtypes of the receptor tyrosine kinase VEGFR, is involved in mediation of almost all cellular responses to VEGF.
2. Signal Transducer and Activator of Transcription 3 (STAT3), acts as a transcription activator, and is phosphorylated by receptor associated kinases in response.