Automated image screening of zebrafish embryos exposed to developmental toxins

In this application note, BioTek demonstrates high-throughput screening and analysis of zebrafish embryos treated with several developmental toxins. Here, BioTek exposes embryos to ethanol, retinoid acid, and cyclopamine, then capture brightfield images of the embryos in multi-well plates with Lionheart™ Automated Microscope. The images are analyzed for size and shape using Gen5™ software. The combination of high-throughput imaging and analysis using a vertebrate model makes this an attractive method for toxicology screening.