Automated Platform Buffer Screening for Multiple Proteins on Big Tuna

Big Tuna was developed to address gaps in low-volume, high-throughput buffer exchange. Big Tuna uses a pressure-based ultrafiltration/diafiltration (UF/DF) method to remove buffer. During the pressure-based filtration, the plate is gently mixed, ensuring that protein cannot accumulate at the membrane surface, while keeping flow more uniform and faster than dead end filtration methods. Big Tuna automates the buffer exchange process, reduces hands-on time and increases throughput. Big Tuna also enables concentration to a new target after the exchange is complete.