Automation of 3D Spheroid Production, Cell Culture and Analysis

Reproducible cell seeding and reliable formation of similar-sized 3D microtissues are essential to enable collection of robust data when adapting more biologically relevant, complex 3D models to high-throughput workflows. This application note demonstrates a standardized, cost effective and automated means of producing and characterizing 3D spheroid microtissues, using the InSphero GravityPLUS™ Hanging Drop System and the Zephyr® G3 automated workstation to automate the liquid handling steps involved in microtissue seeding and transfer to the GravityTRAP™ Plate for long-term culture and imaging