Automation of HTRF® elF4E Kinase Assay Using a 3D Tumoroid-Based Cell Model

This application note demonstrates an in vitro microplate assay that can quantify total, as well as phosphorylated eIF4E. The assay was performed wherein cells were cultured using a novel 3D culture system, RAFT™. All dispensing and removal steps were performed by the MultiFlo™ FX Microplate Dispenser.