Automation of Standardized Cytocompatibility Testing using the Liquid Handling Workstation epMotion® 5070

In this application note by Eppendorf, a specific multiplex method combining three commercially available cell-based assays, using the Liquid Handling Workstation epMotion® 5070, is developed to assess the basic compatibility of cell/biomaterial combinations. Considering the fact that a typical cell culture experiment includes two major sources of variation, biological (cells) and technical issues (pipetting), the current approach includes an optimization study followed by a method development phase. Results show that the implementation of the epMotion 5070 multiplexed cell-based method is successful and advantageous for a standardized analysis of cytotoxicity of cell/biomaterial constructs.