Autophagy Analysis Using Object Spot Counting

Autophagy is critical for the maintenance of cellular homeostasis. However, dysregulated autophagy can lead to death of healthy cells and survival of cancerous cells. Here we describe the use of CYTO-ID^® Autophagy Detection Kit in combination with automatedobject-based spot counting to quantitatively assess the effects of starvation and rapamycin on cellular autophagy by determining the size and number of autophagosomes per cell.