BiFC assay for determination of in-vitro protein-ligand interaction

Bimolecular fluorescence complementation (BiFC) assay is a method used to directly visualize protein-protein interaction in cells. Azure Biosystems and the Malchiodi Lab, University of Buenos Aires, Argentina, explore the application of the BiFC assay to the study of in vitro interaction between the Zika virus NS4B protein and its ligand, TBK1. Discover how the Sapphire Biomolecular Imager makes it possible to detect bimolecular fluorescence complementation spots from transfected adherent cells grown in clear plastic well plates.