Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction. This application note introduces large molecule kinetic analysis on Pall ForteBio’s Octet platform using Bio-Layer Interferometry, and discusses options, techniques and considerations for developing and performing successful kinetic characterization assays and interpreting results.