Blotting Techniques Enhanced by the use of Type I Ultrapure Water

Northern, Southern and Western blotting techniques depend on the use of Type I ultrapure water to obtain high quality results. Water used for blotting purposes should be nuclease-, endotoxin- and bacteria-free to protect the DNA, RNA and proteins under investigation. This application note demonstrates how ultrapure water should be used for the preparation of samples, buffers, reagents, gels and rinsing solutions.