Cell engineering in picodroplets

Picodroplet microfluidics is rapidly becoming the technology of choice for single-cell analysis, providing an easy-to-use tool to encapsulate single cells in picolitre-volume aqueous droplets (picodroplets) at high throughput rates of thousands of droplets per second. This powerful and sensitive technique offers significant cost and efficiency advantages. An additional advantage of this approach is that picodroplets provide a cell friendly culturing environment. Depending on the assay and application of interest, cells can be cultured in these picodroplets for a couple hours, to up to several days. The ability to culture the cells for various lengths of time has opened opportunities for a wide range of applications including antibody discovery, cell line cloning and cell engineering. In this application note, Sphere Fluidics provides an overview of how picodroplets can be used in cell engineering.