Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader

This application note highlights the direct optic bottom reading performance of a HTS microplate reader in two different cell-based fluorescence intensity assays from Invitrogen. First, the intracellular calcium response to histamine was measured in HEK 293 cells using the Fluo-4 Direct™ Calcium Assay. Second, arrestin recruitment through activation of the dopamine D1 receptor with dopamine (D1 agonist) was measured using Tango™ D1-bla U2OS cells and a live cell FRET substrate.