Cellular Migration and Invasion Assays

In this application note Fluxion Biosciences use an integrated platform for running biologically relevant cellular assays under shear flow to study cell migration. A chemotaxis study is performed with neutrophilic HL-60 cells responding to stimulation with chemoattractants, fMLP and IL8. In this experiment the compounds are flowed from one inlet and buffer from the second inlet. Chemotaxis is followed using timelapse microscopy. A second assay is carried out to investigate cancer cell invasion into the Matrigel Matrix with model invasive vs. non-invasive cells.