Characterization of the Interaction Between Recombinant Estrogen Receptor Alpha and Transcriptional Coactivator SCR-1 NR-2 Binding Motif

This application note demonstrates an approach based on fluorescence polarization to characterize the interaction of the NR box 2-binding motif of SRC-1 coactivator with a recombinant ERα ligand binding domain. In presence of estradiol, the affinity between those two proteins was 7.5 nM. The affinity value we obtained was within the same order of magnitude as previously reported values that were based on other methods.