Chromatrap® – Targeting the Epigenetic Landscape Alongside Low Abundant Transcription Factor Recruitment

This application note describes the sensitivity, selectivity and reproducibility of solid phase Chromatrap®, as an assay for Chromatin IP from low cell numbers. Using low-input chromatin concentration, the utilization of Chromatrap® to understand the balance between histone methylation marks and low abundance TF recruitment at positive and negative target gene loci is clearly shown. This new tool enables low volume, fast IP from small samples, eliminating bead loss and high background signals which are prevalent in magnetic bead separation techniques. Handling errors are also reduced, enabling robust analysis of chromatin dynamics and their effect on TF recruitment.