Comparing ddPCR with qPCR for the quantification of AAV vector genomes

Developing effective and reproducible gene therapies requires the use of sensitive and robust testing methods, such as Droplet Digital PCR, to validate the quality of the therapeutic product. Bio-Rad's portfolio of ddPCR Viral Quantification solutions offers an accurate, precise, and robust method to measure the viral titer in a sample.

In this whitepaper, Martin Lock, Director, Process Development Gene Therapy Program, University of Pennsylvania, shares data comparing an AAV genome titer assay based on ddPCR with a standard and an optimized qPCR assay for the titration of both single-stranded (ss) and self-complementary (sc) AAV genomes.