Comparison of AF4, AUC and SEC for Monoclonal Antibody Aggregate Quantitation

Protein aggregation has become an important issue in the development and QC of pharmaceutical formulations and novel drugs. This application note describes a comparison of AF4, AUC and SEC for monoclonal antibody aggregate quantitation. The comparison shows, that AF4 is more easy to use than AUC and at the same time provides more accurate results for aggregates than SEC.