Comparison of Digital Chemiluminescent and Film Imaging of Protein Blots

This application note by UVP, LLC. demonstrates the utility of the BioSpectrum® Imaging System for the imaging of chemiluminescent protein blots. The use of chemiluminescent imaging of protein blotting and the sensitivity, accuracy, dynamic range, speed, and simplicity of this procedure is compared to film. Chemiluminescent blots offer high sensitivity, rivaling radiolabeled techniques, and can be easily reprobed without worrying about radioactivity disposal.