Comparison of Photina Luminescent Calcium Mobilization Assays on the FlexStation 3 Reader and FLIPR Tetra System

G protein-coupled receptors (GPCRs) represent one of the most important therapeutic targets in drug discovery research. GPCRs are membrane-localized proteins that play an important role in cell signaling. When a receptor is activated by a ligand, the conformation of the receptor is modified, activating G-proteins inside the cell. An active G-protein has the potential to induce various cascades of intracellular messengers, including calcium.