Comparison of Various Methods of Retrovirus-Mediated Gene Transduction in Human Stem Cells

RetroNectin® (Recombinant Human Fibronectin Fragment) can dramatically increase retroviral and lentiviral transduction efficiency in hard-to-infect cell types and hematopoietic stem cells. This application note presents a comparison of three different methods (RetroNectin Reagent, Polybrene, and Protamine) for measuring the expression of a retrovirally-delivered recombinant fluorescent protein in stem cells.