Converting an ELISA Assay into an Octet Quantitation Assay

ELISA-based assays and Octet quantitation assays share many similarities. Therefore, conversion of a pre-configured ELISA assay to the Octet platform may only require transfer of assay conditions. When assay conditions need re-optimization on the Octet platform, considerations are often similar to those employed in ELISA. The Octet platform’s direct binding assay method is simple, fast and accurate; the multi-step methods offer high sensitivity and expanded dynamic range. The automated assay formats enhance time-to-results and walk-away time and reduce operating expenses.

This application note provides guidance on converting an existing ELISA-based assay to an Octet assay.