Culture and Assay Systems Used for 3D Cell Culture

This application note will review the general principles, advantages and current limits of in vitro 3D cell culture systems, covering cell biology, tissue modelling and drug discovery and toxicity. The most commonly used matrices for in vitro 3D culture such as ECM-based hydrogels and their applications will also be covered. 3D cell culture methods have been developed over the years to address the limitations of 2D cultures, particularly their physiological irrelevance and inability to emulate in vivo conditions.