Current Standard Protocols for the Illumina Genome Analyzer Sequencing Platform Employ Laborious, Costly, and Unreliable Methods for Quantifying DNA Libraries

Next-generation sequencing (NGS) workflows continue to evolve at an unrelenting pace, driven by users striving for greater reliability, throughput, and efficiency. This case study states that the standard methods for quantifying Illumina GA DNA libraries have a number of important disadvantages. Implementation of the KAPA Library Quantification Kit in sequencing workflows resulted in a significant reduction in variability across multiple libraries.