Cytogenetic Monitoring of Cancer Progression in Circulating Tumor Cells Utilizing the IsoFlux System and GenASIs Spectral Imaging Technology

Fluorescence in situ hybridization (FISH) has emerged as a clinically-useful tool for understanding patient-specific traits of cancer at the molecular level. FISH is typically performed on biopsied tissue samples obtained during surgery. Circulating tumor cells (CTCs) have emerged as a potential source of clinically-useful information that can be accessed with far greater frequency than tissue biopsies. In addition to discussing the current challenges for running FISH on CTCs, this application note presents a complete method for CTC analysis.