Cytotoxicity Studies on 3D Primary Liver Microtissues Using the Operetta System from PerkinElmer

This application note presents a proof of concept study to demonstrate the feasibility of a 4-color toxicity assay using living Troglitazone and Staurosporine treated primary human liver microtissues. In this assay, the combination of High Content Imaging with Multimode Detection using the Operetta system from PerkinElmer provides complementary readouts for assessing phenotypic changes and potential toxicological pathways beyond general cytotoxicity markers.