Defining Statistical Variation Between the Small Molecule Metabolite Profiles of Different Fish Species

This application note investigates tissue extraction techniques to optimize a simple and fast procedure which provides characterization of the small metabolite profiles between two different fish species. The experimental research conducted explores the differences between the metabolite profiles of N-(t-butyldimethylsilyl)-Nmethyltrifluoroacetamide (MTBSTFA) derivatized muscle tissue from different fish species analyzed by GCxGCTOFMS. GCxGC-TOFMS provides increased peak capacity and resolution for the chromatographic separation while fast TOFMS acquires the mass spectral data density necessary to characterize complex biological samples.