Detailed glycosylation analysis of therapeutic enzymes using µPAC capLC-MS and all-ion fragmentation

In this application note, the use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting peptides were separated on a 50 cm μPAC™ capLC C18 column operated at low μL/min flow rate. Glycopeptide peaks were then selectively detected and identified by Orbitrap mass spectrometry (MS) operated in full MS/all-ion fragmentation (AIF) mode.