Detection and Quantitation of Protein with ScanLater Western Blot Detection System

This application note reports on a novel system for Western Blot membrane protein analysis that is incorporated into SpectraMax® i3 and Paradigm® Multi-Mode Microplate Readers. An extended dynamic range due to reduced background is demonstrated, as well as stability of detection over time and number of reads. In addition, a comparison of Scanlater™ Western Blot System to a chemiluminescence method demonstrates improved sensitivity.