Detection of Human Immunoglobulin G (IgG) Using the Infinite F200 PRO’s New AlphaLISA Function

AlphaLISA is a homogeneous, no-wash alternative to conventional ELISAs based on PerkinElmer’s bead-based Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology. AlphaLISA assays can be set up as sandwich or competitive immunoassays to detect and quantify molecules of interest in biological samples. AlphaLISA assays are less prone to signal interference at wavelengths of <600 nm and the use of dedicated AlphaLISA optics permits the analysis of target molecules in blood and serum by drastically reducing the effect of hemoglobin within a sample. This application note describes the implementation of the AlphaLISA technology onto the Infinite F200 PRO, and describes the use of the reader for the detection of human immunoglobulin G (IgG) using PerkinElmer’s AlphaLISA Human IgG Assay Kit.