Development of a Magnetic Bead-based Multiplex Immunoassay for Metabolic Biomarkers in Normal and Diabetic Mouse Serum and Plasma Samples

Obesity and diabetes are on the rise and constitute a series of complex metabolic disorders with complex interactions among multiple proteins and environmental factors. Murine diabetes and obesity models exist to study these disorders through the measurement of circulating metabolic biomarkers, however, the limited amount of sample poses a challenge to effective analysis. This poster describes a novel multiplex immunoassay that can simultaneously measure eight of the most commonly measured metabolic biomarkers, using the Bio-Plex suspension array system.