Differential Cancer Biomarker Secretion of Cultured Human Cells

9 Mar 2012

Diagnostic biomarkers are a key element in cancer research. Research has focused primarily on intracellular biomarkers, such as HER2 or BRAC1, that identify specific tumors, or provide genetic or phenotypic information that can clarify the process of oncogenesis. Using a 22-plex Cancer biomarker assay kit from EMD Millipore this application note describes how the secretion of cancer biomarkers by several human tissue culture cell lines under normal and low serum conditions is characterized. In addition to the established cell lines, three mesothelioma cell lines included two cell lines derived from different individuals were characterized.

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