Direct Comparison of Fast Response and Slow Response Membrane Potential Dye Using the FDSS6000

Ion transport proteins including channels, transporters, and pumps play central roles in cellular bioenergetics, excitability, and a multitude of other biological functions. This application note discusses cell-compatible fluorescent probes, functional assays, and FDSS instrumentation that are used to monitor real-time target activity and screen large chemical libraries for potent and selective modulators. High throughput screening (HTS) compatible, rapid kinetic assays are emphasized, in particular the use of slow response and fast-response voltage-sensitive membrane potential probes to assay ion channel activity in single cells and 96/384-well formats.