Direct PCR from Plant Material is a Challenging Application as a Result of the Diversity of Plant Tissue Types and the Potent PCR Inhibitors Contained within the Tissue

Success rates with direct PCR are typically low due to the high concentrations of inhibitors present in even small amounts of plant material, and the precise control of sample size required. This Application Note demonstrates a systematic approach for reaction optimization with the KAPA3G Plant PCR Kit, as well as a robust method for successful crude sample PCR.