Discriminating Heparin from Chondroitin Sulfate by Charge:Mass Ratio

In this study, heparin from two suppliers is compared, including one lot modified with super-sulfated material. Characterizing key parameters of different heparin lots requires multiple techniques. Weight-average molar mass measured by multi-angle static light scattering (MALS) and hydrodynamic radius measured by dynamic light scattering (DLS) can be used to characterize the size and polydispersity of the molecule. These quantities can also be used for lot-to-lot comparison and to qualify heparin from different suppliers.