Dynamic Fielding for Syngene Image Capture Systems

Some manufacturers use a Flat Field Correction method in order to address uneven illumination of light sources. This involves subtracting the image information from an empty field of view or ‘perfectly flat’ fluorescent reference sample from that of the same field of view with the gel added. As this process involves the subtraction of one image from another, the integrity of the raw data of the initial captured image is compromised. Such manipulation is incompatible with Good Laboratory Practice (GLP). Syngene uses the Dynamic Fielding Correction method to address uneven light illumination whilst maintaining GLP compliance.