Efficient Delivery of Active Cas9 Protein and Target-Specific sgRNA to a Broad Range of Cell Types

The Guide-it CRISPR/Cas9 Gesicle Production System is a complete and simple methodology for creating cell-derived nanovesicles that deliver active Cas9 protein complexed with a gene-specific sgRNA of your own design. Due to the non-persistence of Cas9, gesicles leave no additional footprint, reducing potential off-target effects. Gesicles are nontoxic and include surface proteins that mediate binding and fusion with the cellular membranes of target cells. These critical features enable efficient Cas9 delivery and gene editing in a broad range of cell types, discover more in this application note.