Efficient Expansion of Suspension CHO Cells in Corning® 5 Liter Erlenmeyer Flasks

Erlenmeyer flasks are commonly used for expansion of a variety of suspension cell lines for bioprocessing applications. This study describes a protocol to scale up suspension CHO cells from 40 mL to 15L of total volume in just 12 days using Corning 5L Erlenmeyer flasks. It is also demonstrated that the Corning 5L Erlenmeyer flasks provide a more favorable culture environment (higher oxygenation and lower lactate metabolite accumulation) and support a higher yield of viable CHO cells when compared to competitor 5L Erlenmeyer flasks.