Enhancing Throughput with Multiple Cell Lines Per Well with the QPatch HTX

In this application note, a series of experiments with a combination of ion channels involved in cardiac risk assessment are conducted. Parallel operation of 48 multi-hole patch clamp sites, each comprising 10 individual patch clamp holes, in a single measurements site, allows for high throughput. The ion channel currents were separated temporally or pharmacologically by proper choices of voltage protocols or ion channel inhibitors. Biophysical and pharmacological data are presented. Simultaneous recordings on multiple cell lines expressing different ion channels are analysed using the QPatch HTX.