Evaluation of Bioruptor in Shotgun Library Preparation for Massively Parallel Sequencing on Illumina Genome Analyzer II

The Illumina Genome Analyzer II (GA-II) performs massively parallel short-read DNA sequencing, with the potential to generate up to 10 gigabases in a single run. The technology provides researchers with a significantly cheaper alternative to conventional sequencing by capillary electrophoresis. A consistent and reliable method of DNA fragmentation is essential in Illumina library construction. This application note evaluates and compares two types of fragmentation methods: nebulization and shearing using the Bioruptor.