Expansion of Mouse Embryonic Stem Cells in Suspension Culture Using CultiFlask 50 Disposable Bioreactors

In this application note, disposable CultiFlask 50 bioreactors were evaluated for their suitability to expand undifferentiated mouse ES cells in long term suspension culture. Proliferation and viability values of ES cells, throughout several passages, were assessed. After the expansion phase an undifferentiated state was verified by flow cytometric measurement of pluripotency marker (SSEA-1 and Oct-3/4) expression. In addition, after induced differentiation, pluripotency marker expression and cardiogenic differentiation was analysed.