Expansion of Primitive Hematopoietic Progenitor Cells

Hematopoietic Stem Cells (HSC / HPC) represent a heterogeneous population of primitive blood progenitor cells that fall into several different classes. Within the human body, they are mainly located in the bone marrow of adults, but are also found in various fetal tissues, e.g. umbilical cord blood, placenta and fetal liver. It is known that in vivo HSC are able to expand to very large numbers of daughter cells by virtue of their pronounced self-renewal abilities. However, to date researchers faced difficulties during in vitro expansion of undifferentiated HSC, with increasing differentiation of the cells. This application note demonstrates the use of PromoCell HPC Expansion Medium DXF to provide a more consistent culture environment.