FLIPR Penta high-throughput cellular screening system

In this application note, Molecular Devices^® describes the anatomy and applications of its FLIPR^® Penta high-throughput cellular screening system.

The applications of this system include:

• GPCR: Monitor GPCR activity and corresponding intracellular second messenger changes (calcium and cAMP) using fluorescence and luminescence cell based assays
• Membrane potential: Observe real-time membrane potential changes associated with ion channel activation and ion transporter proteins
• Cardiomyocytes: Observe calcium flux to assess cardiotoxicity in the early stages of drug discovery
• Potassium channels: Measure functional activity of ligand- and voltage-gated potassium channels
• Ligand-gated calcium channels: Measure the change of global intracellular calcium, in response to the activation of ion channels
• 3D assays: Detect calcium oscillations in 3D cardiomyocyte and neuronal, providing a biologically relevant assay system to assess potential new pharmaceutical treatments and environmental toxins
• Biochemical assay: Enable fast 'inject and read' kinetic enzyme assays, increasing the throughput of biochemical assays compared to standard plate readers