Fura-2 QBT Calcium Kit from Molecular Devices: A New Homogenous Fura-2 Calcium Assay

Fura-2 dye has long been considered an important tool to measure calcium mobilization in cellular imaging,
GPCR-mediated intracellular calcium flux, and ion channel activation. This application note demonstrated the use of the Fura-2 QBT™ Calcium Kit from Molecular Devices® which incorporates proven quench based technology with a ratiometric Fura-2 calcium indicator to provide a homogenous assay to minimize cell based variability, while increasing throughput through elimination of cell washing prior to detection. In addition, the Fura-2 QBT Calcium Kit excites in the ultraviolet spectrum, enabling researchers to mitigate fluorescent compounds that are excited at 488 nm.