Gene Expression II Relative Quantification

The aim of this Application Note is to highlight the potential drawbacks of using single housekeeping genes in relative quantification analysis using the ΔΔCT method. The multiple housekeeping gene approach using geNorm is presented as the preferred method against which relative quantification results from different methods are compared. The benefits of using the novel KAPA SYBR® FAST qPCR Kit in the context of gene expression analysis using multiple housekeeping genes are also highlighted.