Generation of Multiple Images from a Single Tissue Section with Dual Polarity Desorption Electrospray Ionization Mass Spectrometry

This application note demonstrates that by optimizing DESI conditions, a wealth of molecular information can be accessed from a single tissue section, without the need to substantially alter the analysis conditions. Mass spec imaging of tissue sections was accomplished by using a SYNAPT® G2-Si HDMS Mass Spectrometer equipped with a 2D-DESI source. Data was generated and analyzed using HDI Software v1.3.