Growing CHO Cells in a New Brunswick™ CelliGen® BLU Benchtop, Stirred-Tank Bioreactor Using Single-Use Vessels

This study presents a typical protocol for the setup and operation of the Eppendorf New Brunswick CelliGen BLU single-use, stirred-tank bioreactor, a versatile new benchtop system for the culture of a wide range of mammalian cells. A procedure for culturing Chinese Hamster Ovarian (CHO) cells in a 5.0 L vessel, using CD CHO serum-free medium in a batch culture is described.