Guide for Successful Chromatin Preparation using the Bioruptor® Pico

Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in cells and tissues. The assay can be cumbersome and the success is highly dependent on the quality of chromatin. The first critical step of a successful ChIP experiment is the preparation of sheared chromatin which is representative of the biological scenario of interest. In this comprehensive guide, learn to optimize cross-linking, choosing appropriate lysing and sonication strategies and adapt your process to your application.