High-throughput DNA library preparation for nanopore sequencing using DreamPep NGS and ligation sequencing KIT XL v14

In this application note, Oxford Nanopore Technologies describes how it has developed a sequencing protocol for the analysis of longer DNA fragments. This approach provides comprehensive genomic insights, including the detection of structural variants, copy number variations, and methylation patterns. Obtaining complete genome information is essential for understanding chromosome function, human diseases, and genomic variation. The integration of the Ligation Sequencing Kit XL V14 on the DreamPrep NGS workstation promises a reliable and automated solution for high-throughput, long-read DNA library preparation. The resulting libraries demonstrate comparable quality to those produced manually and are compatible with Oxford Nanophore Technology sequencing devices sequencing devices.