High-Throughput Screening Data Analysis for Cell-Based Assays Using Corning® Epic® Technology: Going Beyond Simple Hit/Non-Hit Classification with Epic Signal Identification (SI)

High-throughput screening (HTS) in cell-based assays is used to identify lead compounds for a wide range of therapeutic areas. Compound interaction with targets results in dynamic mass redistribution (DMR) in the cell that is detectable by Corning Epic Technology. Using either kinetic or HTS Profiling approaches, Epic Technology enables the extraction of additional information extending beyond simple “hit/non-hit” analysis. This document presents internal benchmarking data for the Epic SI tool performed on a commercial library and data from a customer screening project, which illustrate the value of Epic SI analysis.