High-throughput screening of 3D cell cultures with multiple high density scaffold-free spheroids for cancer toxicity studies

In this application note, Molecular Devices demonstrates the use of the Elplasia 96-well plates with a 3D culture workflow that includes spheroid generation, compound treatment, cytotoxicity assay, high-content imaging on the ImageXpress^® Micro Confocal High-Content Imaging System, and 3D image analysis using MetaXpress^® High-Content Image Acquisition and Analysis Software. The ability to easily generate multiple spheroids and to seamlessly integrate the workflow with high-content imaging promises to have significant applications in drug discovery and compound toxicology.